The Effect of Support of Zinc and Melatonin on Lipid peroxidation of Various Tissues in Rats with Breast cancer induced by DMBA

Abstract

The aim of the present was study determine the effect of zinc and melatonin supplementation on lipid peroxidation of various tissues in female rats with breast cancer induced by DMBA (7,12- Dimethylbenz[a]anthracene). Total 42 Wistar albino female rats were equally separated 5 groups. Group 1 control, Group 2 DMBA control, Group 3 DMBA+Zinc, Group 4 DMBA + Melatonin, Group 5 DMBA+Zinc+Melatonin. Lung, liver, spleen, pancreas and kidney tissue of rats were examined for MDA and GSH by spectrophotometric. The highest lung, liver, spleen, pancreas and kidney MDA levels were determined in group 2 (p<0.05). DMBA+Zinc, DMBA+Melatonin and DMBA+Zinc+Melatonin groups have lower MDA levels compared to group 2 (p<0.05). Similarly, zinc and melatonin supplemented groups (groups 3, 4 and 5) have higher GSH levels compared to cancer induced group (group 2) (p<0.05). The results of present study show that increased damage in lung, liver, spleen, pancreas and kidney tissues are inhibited by zinc/melatonin or zinc+melatonin supplementation. Combine zinc and melatonin supplementation may contribute to preventing of lipid peroxidation in tumoral conditions.