A Novel Immobilization Matrix For The Biosensing Of Phenol: Self Assembled Monolayers Of Calixarenes
Tarih
2017-04-01Yazar
TAŞÇI, Filiz
SAYIN, Serkan
AĞ SELECİ, Didem
DEMİR, Bilal
AZAK, Hacer
YILDIZ, Hüseyin Bekir
ODACI DEMİRKOL, Dilek
TİMUR, Suna
Üst veri
Tüm öğe kaydını gösterÖzet
Aim: The development of calixarene based phenol biosensor.
Methods: This study describes the application of a calixarene derivative, 5,17-diamino-25,27-bis(3-thiol-1-oxypropane)-26,28-dihydroxycalix[4]arene (HS-Calix-NH2) which has both amino and thiol functionalities, in the practical surface modifications for biomolecule binding. The structure of HS-Calix-NH2 allows easy inter-action with Au surface and one-step biomolecule immo-bilization. Self-assembled monolayers (SAMs) of p-amino-functionalized mercaptoalkylcalixarene (HS-Calix-NH2) were formed onto the Au electrode. Then, Laccase (Lac) nzyme was immobilized onto the modified surface by crosslinking with glutaraldehyde (GA). Resulted electrode (HS-Calix-NH2/Lac) was used for the electrochemical anal-ysis of phenolic compounds at − 50 mV.
Results: The linearity was observed in the range of 0.1–100 μM and 1.0–100 μM for catechol and phenol, respectively. The potential use of the biosensor was inves-tigated for phenol analysis in artificial samples which simulate the industrial waste water, which is highly acidic and composed of concentrated salt, without needing any sample pre-treatment step.
Conclusion: The prepared Lac biosensor has a potential for rapid, selective and easy detection of phenolic con-taminations in samples.
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