Construction of an Amperometric Cholesterol Biosensor Based on DTP(aryl)aniline Conducting Polymer Bound Cholesterol Oxidase

Abstract

In this study, an amperometric cholesterol biosensor was constructed based on cholesterol oxidase immobilized on a conducting 4-(4H-dithienol[3,2-b:2’,3’d]pyrrole-4)aniline polymer, (DTP(aryl)aniline). Glassy carbon electrodes were covered with P(DTP(aryl)aniline) which is used for the wiring of enzyme to the electrode surface by using electro-polymerization. The electron transfer was successfully made by the bio-catalytic activity and possession of the unique morphology of the polymer allowed efficient immobilization of the cholesterol oxidase enzyme. Analytical performances, linear range, detection limit, limit of quantification and the Michaelis-Menten constant (Km) of biosensor electrodes were obtained 2.0 mM–23.7 mM, 0.27 mM, 0.82 mM, 17,81 mM respectively. Biosensor optimization parameters: optimum pH, optimum temperature, stability test and response time were evaluated. The real sample and recovery studies were also performed in order to show applicability of the biosensing electrodes