Effects of Resveratrol on In Vitro Stomach Smooth Muscle Contraction in Rats with Hepatocellular Carcinoma

Abstract

AIM: Hepatocellular carcinoma (HCC) is the fifth most common cancer in the world. Resveratrol is a polyphenol that has antiinflammatory, antiproliferative, antitumoral effects. There are studies on relaxant effects of resveratrol in various smooth muscles such as aorta, gallbladder, corpus cavernosum. In this study, we aimed to determine the effect of resveratrol on gastric smooth muscle contraction in HCC. METHODS: 3 groups were formed using Wistar albino male rats: 1) Control Group (n=8) were injected 0.9 % NaCl intraperitoneally once daily for 80 days. 2) Diethylnitrosamine (DEN) group (n=8), DEN (100 mg/kg) was injected intraperitoneally once per week for 9 weeks and HCC was generated. 3) DEN+Resveratrol group (n=8), following DEN administration, resveratrol (100 mg/kg/day) was injected intraperitoneally for 14 days. Animals were sacrificed under anesthesia, then the stomachs were removed and placed in Krebs solution. The liver was histopathologically examined for tumor formation and HCC was detected. 2 x 10 mm strips were prepared longitudinally from each animal's stomach smooth muscle tissue. Muscle strips that applied 2 g tension were washed with 15 minute intervals and allowed to equilibrate for 1 hour in isolated organ bath. Then, contractions were induced by 10-5 M acetylcholine (Ach). Spontaneous contractions, Ach-induced contractions, and 10 minute plateau amplitudes of muscle strips were recorded. One way ANOVA was used for statistical evaluation. RESULTS: There was a significant increase in Achinduced contractions in DEN group (3505.21±268.05) compared with control group (2440.32±268.05) (p<0.05). Spontaneous contractions (974.95±124.80), Ach-induced contractions (1741.17±268.05) and 10 minute plateau amplitudes (1468.85±234.17) of DEN +Resveratrol group were decreased compared with DEN group (1731.23±124.80; 2440.32±268.05; 2107.58±234.17) (p<0.05). CONCLUSION: This study suggests that resveratrol has a relaxing effect in gastric smooth muscles of HCC-induced rats.