The Cytotoxic Effects of Soybean (Glycine max L.) in Breast Cancer MCF-7 Cell Line In Vitro

Abstract

Aim of the study: Breast cancer is one of the most noticeable disease in women aged 20-59 years also it is expected to account for 30% of all new cancer diagnoses in women in 2016, Long-term use ofsoybean (Glycine max 1.) may prevent the progression of cancer. However, the molecular mechanism forthe functions of soybean remains unclear. So we decided to see how effective soybeans are on MCF-7Cell lines. Materials and Methods: Human breast cancer cell lines MCF-7 were treated with different concen-trations of methanol extract of soybean seeds. The growth rate was evaluated by XTT tests for 48 and72 hours. Results and Discussion: Soybean showed significant inhibitory effects on the growth rates of MCF-7 cells in a concentration-dependent way (P<0.05). Best efective concentration was 131.65 uM for the 72hours plates on MCF-7 cell lines. result of 48 hours soybeans concentration was calculated as a 476.8 uM. Cytotoxicity assays resulted in a dose dependent growth inhibition of MCF-7 cells. The different concen-tration of soybean will be beneficial to the therapy of breast cancer since its causes may be involved inmultiple aspects. Therefore, soybean methanol extract will necessitate further deep investigation for aprobable use as a cancer preventive food additive.